 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Cryoprotectants on Cryopreservation of Sugarcane Cells ) ผู้เขียน: Sonthichai Chanprame,  ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์ , Chongkolnee Weerasathakul สื่อสิ่งพิมพ์:pdf AbstractCell suspension of sugarcane cultivar ROC3 treated with different concentration and combination of cryoprotectant were cryopreserved. Before cryopreservation, temperature of cell suspension was reduced from room temperature to O?C/ min. and from O?C to -40?C at the rate of 0.5?C/min.. Then, cell suspension were storaged in liquid nitrogen for 30 minutes. After storage, sugarcane cells were recultured again on solid media. Sugarcane cells treated with 15% dimethysulphoxide (DMSO) and 1.OM sucrose showed the best regrowth among the cryoprotectants studied. |